Method for the synthesis of a bifunctional complex

摘要

Disclosed is a method for obtaining a bifunctional complex comprising a display molecule part and a coding part, wherein a nascent bifuntional complex comprising a chemical reaction site and a priming site for enzymatic addition of a tag is reacted at the chemical reaction site with one or more reactants, and provided with respective tag(s) identifying the reactant(s) at the priming site is using one or more enzymes.

主权项

1. A method for obtaining a library comprising bifunctional complexes, wherein each complex comprises a display molecule part and coding part, said method comprising the steps of:
(a) reacting a nascent bifunctional complex comprising a chemical reaction site and a priming site at the chemical reaction site with one or more reactants; and (b) reacting the priming site with one or more tags identifying the one or more reactants, the reaction being catalyzed by an enzyme; wherein steps (a) and (b) provide an intermediate bifunctional complex having a modified chemical reaction site containing a structural entity formed from the nascent bifunctional complex and the one or more reactants and a modified priming site containing the one or more tags; (c) reacting the intermediate bifunctional complex with one or more additional reactants at the modified reactant site and one or more additional tags identifying the one or more additional reactants at the modified priming site using the methods of steps (a) and (b); (d) optionally repeating step (c) as many times as desired; and (e) obtaining a library of different bifunctional complexes; wherein the library is produced using split and mix methods, and wherein the coding part comprises an identifier oligonucleotide comprising tags identifying the reactants which have participated in the formation of the display molecule, and a reactant and the tag identifying the reactant are not linked prior to their reaction with the chemical reaction site and the priming site, respectively, of the nascent bifunctional complex.