Methods and means for stem cell proliferation and subsequent generation and expansion of progenitor cells, as well as production of effector cells as clinical therapeutics

摘要

The invention provides means and methods for stem cell proliferation and subsequent generation and expansion of progenitor cells. The invention in particular provides media and other culture conditions for the same. The cells are preferably used as effector cells as clinical therapeutics.

主权项

1. A medium for culturing, expanding and/or differentiating stem cells, said medium comprising a basic cell culture medium, 1-20% human serum in relation to the volume of culture medium, 2-10 mmol/l O-acetyl-L-carnitine in relation to the volume of culture medium, 40-80 mg/l N-desulphated-N-acetylated heparin N-desulphated-N-acetylated heparin in relation to the volume of culture medium and a combination of cytokines.