| 摘要 |
<p>The invention relates to a polypeptide comprising an amino acid having a bicyclo[6.1.0]non 4- yn -9- ylmethanol (BCN) group, particularly when said BCN group is present as: a residue of a lysine amino acid. The invention also relates to a method of producing a polypeptide comprising a BCN group , said method comprising genetically incorporating an amino acid comprising a BCN group into a polypeptide. The invention also relates to an amino acid comprising bicyclo[6.1.0]non 4 -yn- 9- ylmethanol (BCN) particularly and amino acid which is bicyclo[6.1.0]non 4- yn- 9- ylmethanol (BCN) lysine. In addition the invention relates to a PylRS tRNA synthetase comprising the mutations Y271M, L274G and C313A.</p> |
