METHODS FOR SCORING GENE COPY NUMBER IN BIOLOGICAL SAMPLE USING IN SITU HYBRIDIZATION

摘要

PROBLEM TO BE SOLVED: To provide methods for predicting prognosis of a neoplastic disease (lung cancer, for example NSCLC), including determining the IGF1R gene copy number in a biological sample from a patient having a neoplastic disease.SOLUTION: Methods of scoring copy number of a gene of interest in a biological sample include identifying individual cells in the sample having highest number of signals for the gene of interest detected by in situ hybridization, counting the number of signals for the gene of interest in the identified individual cells, and determining an average number of signals per cell. An increase in IGF1R copy number predicts a good prognosis of the neoplastic disease in the patient.