| 主权项 |
1. A method of preparing a fibrin composition, the method consisting of:
contacting a first portion of an anticoagulated whole blood, a fraction of the anticoagulated whole blood, or a component of the anticoagulated whole blood with a contact activation agent and an extrinsic coagulation pathway initiation agent to provide a coagulated mass in less than about 30 minutes; extracting thrombin from the coagulated mass to provide a thrombin composition; fractionating a second portion of the anticoagulated whole blood, the fraction of the anticoagulated whole blood, or the component of the anticoagulated whole blood to obtain a fibrinogen composition and a platelet plasma composition; contacting the fibrinogen composition, platelet plasma composition, or a combination thereof with a recombinant protein composition; contacting the thrombin composition with a stabilizing agent to provide a thrombin composition having a table-life of more than about 6 hours, wherein the anticoagulated whole blood, a fraction thereof or a component thereof is not contacted with the stabilizing agent during the generation of the coagulated mass; contacting the thrombin composition, the fibrinogen composition and the platelet plasma composition to generate a fibrin composition; and adding at least one micro-particle to the fibrin composition, wherein the at least one micro-particle includes at least one of the following: a pharmaceutical agent, a therapeutic agent, a medical agent, a biological agent, and any combination thereof; adding at least one protecting group to protect one or more functional groups of at least one of the following: the thrombin composition, the fibrinogen composition, the platelet plasma composition, and the fibrin composition, wherein the protecting group includes at least one of the following: a carbonyl protecting group, an alcohol protecting group, and an amine protecting group. |
