DIAGNOSTIC ASSAY FOR ALZHEIMER'S DISEASE

摘要

The present invention relates to the field of Alzheimer's disease (AD). More specifically, the present invention provides methods and compositions useful in diagnosing and assessing AD. In another embodiment, a method for diagnosing Alzheimer's Disease (AD) in a patient comprises the steps of (a) obtaining CSF sample from the patient; (b) adding to the sample isotopically labeled peptides identical to a portion of one or more biomarker proteins, wherein the one or more biomarker proteins comprise total APP, APP isoform 751, APP isoform 770, amyloid beta 40 peptide, APOE, and Clusterin; and (c) measuring the one or more biomarker proteins in the sample using mass spectrometry, wherein the level of one or more biomarker proteins compared to a control correlates with AD in the patient.

主权项

1. A method for diagnosing Alzheimer's Disease (AD) in a patient comprising the steps of:
a. measuring one or more biomarker proteins using mass spectrometry on a cerebrospinal fluid sample taken from the patient, wherein the one or more biomarker proteins comprise total amyloid precursor protein (APP), APP isoform 751, APP isoform 770, amyloid beta 40 peptide, apolipoprotein E (APOE), and Clusterin; and b. correlating the measured proteins to AD in the patient based on the level of the one or more biomarker proteins or a ratio between two or more fragments thereof.