发明名称 |
ANTHROPOGENIC INSECT-RESISTANT GENE AND CRY1C TOXIN IDIOTYPE SINGLE-CHAIN ANTIBODY ENCODED THEREBY AND APPLICATION THEREOF |
摘要 |
An anthropogenic insect-resistant gene having a nucleotide sequence represented by SEQ ID NO.1, and a Cry1C toxin idiotype single-chain antibody encoded by said anthropogenic insect-resistant gene and having an amino acid sequence represented by SEQ ID NO.2; the antibody is a β-type and has insecticidal activity, and after expression by the prokaryotic system, the primary culture thereof has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV; the β-type Cry1C toxin idiotype single-chain antibody of the present invention is obtained without animal immunisation, has a short preparation period and small amino acid sequence, and is suitable for large-scale in vitro production. The present invention is an entirely new insect-resistant gene resource, and has significant implications for decreasing the various safety risks associated with the widescale use of existing Bt toxins, substituting Bt toxins in the biocontrol of agricultural pests, and reducing the use of pesticides. |
申请公布号 |
WO2015109953(A1) |
申请公布日期 |
2015.07.30 |
申请号 |
WO2015CN70422 |
申请日期 |
2015.01.09 |
申请人 |
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES |
发明人 |
LIU, XIANJIN;XU, CHONGXIN;ZHANG, XIAO;LIU, YUAN;XIE, YAJING;ZHANG, CUNZHENG;YU, XIANGYANG;WANG, DONGLAN |
分类号 |
C12N15/13;A01N47/44;A01P7/04;C07K16/12;C12N15/70 |
主分类号 |
C12N15/13 |
代理机构 |
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