PRE-PROCESSING/ELECTROPHORESIS INTEGRATED CARTRIDGE, PRE-PROCESSING INTEGRATED CAPILLARY ELECTROPHORESIS DEVICE, AND PRE-PROCESSING INTEGRATED CAPILLARY ELECTROPHORESIS METHOD

摘要

To allow fully automated implementation of all steps of from pre-processing up to electrophoresis. Provided is a pre-processing/electrophoresis integrated cartridge including one or more block structures corresponding to the individual steps of pre-processing. Each block structure includes (1) a first block having a reaction tank, a reagent tank, a plurality of flow channels that connect the tanks, a plurality of control valves arranged in the flow channels, and a flow channel that connects a reaction tank in a block structure located at a preceding stage and a reaction tank in a block structure located at a next stage, and (2) a second block having a phoretic solution tank, a cathode buffer solution tank, a wash solution tank, a flow channel that connects a carrier tank in the block structure located at the preceding stage and the phoretic solution tank, and a control valve for the flow channel.

主权项

1. A pre-processing integrated capillary electrophoresis device comprising:
a mount table for a pre-processing/electrophoresis integrated cartridge, the cartridge including one or more block structures each corresponding to a step of extracting a nucleic acid from a biological sample, a step of performing a PCR reaction on a target region of the nucleic acid, and a step of performing a cycle sequence reaction on the nucleic acid that is amplified, each block structure including
(1) a first block having a reaction tank into which a biological sample is injected, a reagent tank that stores a reagent for use in the reaction, a plurality of flow channels that connect the tanks, a plurality of control valves arranged in the flow channels, and a flow channel that connects a reaction tank in a block structure located at a preceding stage and a reaction tank in a block structure located at a next stage, and(2) a second block having a phoretic solution tank that stores a phoretic solution, a cathode buffer solution tank that stores a buffer solution containing an electrolyte for use in capillary electrophoresis, a wash solution tank that stores a wash solution for washing a tip end of a capillary to avoid contamination between samples, a flow channel that connects a carrier tank in the block structure located at the preceding stage and the phoretic solution tank, and a control valve for the flow channel; a temperature adjustment mechanism for adjusting a temperature of a solution in the cartridge; a capillary electrophoresis mechanism; and a controller configured to control opening and closing of the control valves to control liquid transfer in and between the first and second blocks, and control an electrophoresis operation by controlling the capillary electrophoresis mechanism, wherein at least one of the step of extracting a nucleic acid from the biological sample, the step of performing a PCR reaction on a target region of the nucleic acid, or the step of performing a cycle sequence reaction on the nucleic acid that is amplified, and a capillary electrophoresis step of analyzing the nucleic acid are all executed automatically.