Use of lecithin:retinol acyl transferase gene promoter methylation in evaluating the cancer state of subject

摘要

The present invention relates to a method of evaluating the cancer state of a subject using lecithin:retinol acyl transferase (LRAT) gene promoter methylation status. Methods of analyzing and quantifying LRAT gene promoter methylation level are also disclosed. The present invention also relates to methods of determining the prognosis for s subject having cancer by assessing LRAT mRNA expression and LRAT protein expression. Methods of cancer detection, diagnosis, prognosis, and treatment are also disclosed.

主权项

1. A method comprising:
providing a colorectal cancer sample from a human subject; determining in the provided sample from the subject a first methylation level of lecithin:retinol acyl transferase gene promoter having a nucleotide sequence corresponding to the nucleotide sequence of SEQ ID NO: 1; providing a second methylation level of the lecithin:retinol acyl transferase gene promoter having a nucleotide sequence corresponding to the nucleotide sequence of SEQ ID NO: 1, said second methylation level being from either (1) a colorectal sample obtained from the subject at a time before said determining, or (2) one or more reference samples obtained from early stage colorectal tumors or benign colorectal tissue; comparing the first methylation level of the lecithin:retinol acyl transferase gene promoter nucleotide sequence with the second methylation level of the lecithin:retinol acyl transferase gene promoter nucleotide sequence detecting a decrease in the first methylation level compared to the second methylation level; and administering a pharmaceutical composition comprising a lecithin:retinol acyl transferase inhibitor to the subject based on said detecting.