Modulation of NR2F6 and methods and uses thereof

摘要

The application provides methods of modulating NR2F6 in a cell or animal in need thereof by administering an effective amount of a NR2F6 modulator.

主权项

1. A method for identifying compounds that can modulate the function of NR2F6 in mammalian cells comprising the steps of:
(i) selecting a cancer (a) cell line or (b) cell-free lysate to use; (ii) expressing in the cancer cell-line or cell-free lysate proteins encoded by one or more recombinant DNA vectors that comprise a reporter assay system, having a portion of the gene with SEQ ID NO:1 that has been predetermined to control the transcriptional activity of the gene NR2F6 and that encodes for expression of an amino acid sequence of at least 75% sequence identity to a portion of the amino acid sequence of SEQ ID NO: 2; (iii) contacting the cancer cell-line or or cell-free lysate with a candidate compound; (iv) measuring the ability of the candidate compound to inhibit or stimulate said reporter assay system; and (v) comparing the measurement of the ability of the candidate compound to inhibit or stimulate said reporter assay system with a suitable control so as to determine whether said inhibition or stimulation of said reporter assay system is indicative of a compound for modulating the function of NR2F6 in a mammal.