| 摘要 |
<p>“A method of in-vitro maturation of spermatid, media thereof and method of preparation of media” The present invention describes a method of in-vitro maturation of spermatid, media thereof and method of preparation of media. It particularly relates to a culture the spermatid. The culture media comprises Dulbecco’s Modified Eagle Medium (DMEM), non-essential amino acid, fetal bovine serum (FBS) and penicillin-streptomycin as antibiotics. The testicular biopsy is incubated in culture medium at required temperature for 24-65 hours. This method provides the viable result for non-obstructive azoospermia patients and overcome the infertility in male.</p> |
