| 发明名称 |
INCREASED ISOPRENE PRODUCTION USING THE ARCHAEAL LOWER MEVALONATE PATHWAY |
| 摘要 |
The invention features methods for producing isoprene from cultured cells using a feedback-resistant mevalonate kinase polypeptide, such as an archaeal mevalonate kinase polypeptide. The resulting isoprene compositions may have increased yields and/or purity of isoprene. |
| 申请公布号 |
US2015203873(A1) |
申请公布日期 |
2015.07.23 |
| 申请号 |
US201414323596 |
申请日期 |
2014.07.03 |
| 申请人 |
Danisco US Inc. ;The Goodyear Tire & Rubber Company |
发明人 |
BECK Zachary Q.;CALABRIA Anthony R.;MILLER Michael C.;NIELSEN Alex T.;VAVILINE Dmitrii V. |
| 分类号 |
C12P5/00;C12P9/00;C12N9/88;C12N9/12 |
主分类号 |
C12P5/00 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of increasing the rate or flux of production of 3,3-dimethylallyl diphosphate (DMAPP), isopentenyl diphosphate (IPP), or a product derived from DMAPP or IPP comprising:
(a) culturing cells comprising (i) one or more heterologous nucleic acids encoding feedback-resistant mevalonate kinase polypeptides, and (ii) one or more nucleic acids encoding a mevalonate (MVA) pathway polypeptide, wherein the cells are cultured under suitable culture conditions for increasing the rate or flux of production of DMAPP, IPP, or a product derived from DMAPP or IPP, and (b) producing DMAPP, IPP, or a product derived from DMAPP and/or IPP. |
| 地址 |
Palo Alto CA US |
