| 发明名称 |
HOMOPOLYMER MEDIATED NUCLEIC ACID AMPLIFICATION |
| 摘要 |
According to some aspects of the invention, provided herein are methods of amplifying nucleic acids using homopolymer-dedicated ligation. The methods, in some embodiments, comprise adding a first homopolymer of at least 12 nucleotides to each 3′ end of blunt-ended double-stranded nucleic acid containing a target nucleic acid, thereby producing a partially double-stranded nucleic acid. |
| 申请公布号 |
US2015203887(A1) |
申请公布日期 |
2015.07.23 |
| 申请号 |
US201314423311 |
申请日期 |
2013.08.23 |
| 申请人 |
Tufts University |
发明人 |
Lazinski David W.;Camilli Andrew |
| 分类号 |
C12P19/34 |
主分类号 |
C12P19/34 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method, comprising adding a first homopolymer of at least 12 nucleotides to each 3′ end of a blunt-ended double-stranded nucleic acid containing a target nucleic acid, thereby producing a partially double-stranded nucleic acid having a homopolymer of at least 12 nucleotides at each 3′ end. |
| 地址 |
Medford MA US |
