METHOD AND SYSTEM OF MICROFLUIDIC IMMUNOASSAY USING MAGNETIC BEADS

摘要

A microfluidic Western blot method and system including a microfluidic western blot method for immunoassay of proteins, the method including introducing a sample including the proteins onto a chip (100); electrophoretically separating the proteins; binding the separated proteins to beads to form protein-attached beads, the beads being magnetic; flowing the protein-attached beads into a magnetic holding region (130); applying a magnetic field to the magnetic holding region (130) to fix the protein-attached beads in place within the magnetic holding region (130); binding primary antibodies to target proteins on the protein-attached beads; binding secondary antibodies to the bound primary antibodies; and detecting the bound secondary antibodies.