| 主权项 |
1. A system that generates a concentration gradient eluent flow for a chromatographic separation, the system comprising:
A) a pump configured to pump a liquid; B) an electrolytic eluent generating device configured to generate at least two different generants where a first generant concentration and a second generant concentration are both controllable as a function of time, the electrolytic eluent generating device comprising;
a) a first eluent generator configured to
i) receive the liquid from the pump;ii) add a first generant and a first gas byproduct to the liquid; andiii) output the liquid that includes the first generant and the first gas byproduct, in which the first eluent generator comprises:iv) a first ion source reservoir including a source of cations or anionsv) a first generation chamber including an inlet configured to receive the liquid and an outlet configured to output the liquid that includes the first generant and the first gas byproduct;vi) a first ion exchange barrier at least partly disposed between the first ion source reservoir and the first generation chamber;vii) a first electrode in electrical communication with the first ion source reservoir; andviii) a second electrode in electrical communication with the first generation chamber;b) a second eluent generator configured to
i) receive the liquid from the first eluent generator;ii) add a second generant and a second gas byproduct to the liquid; andiii) output the liquid that includes the first generant, the second generant, the first gas byproduct, and the second gas byproduct, in which the second eluent generator comprises:iv) a second ion source reservoir including a source of cations or anionsv) a second generation chamber including an inlet configured to receive the liquid and an outlet configured to output the liquid that includes the second generant and the second gas byproduct;vi) a second ion exchange barrier at least party disposed between the second ion source reservoir and the second generation chamber;vii) a third electrode in electrical communication with the second ion source reservoir; andvii) a fourth electrode in electrical communication with the second generation chamber; and C) an electronic circuit configured to
a) apply a first controlling signal to the first eluent generator to generate the first generant concentration, andb) apply a second controlling signal to the second eluent generator to generate the second generant concentration, where at least one of the first or second controlling signals is increased or decreased as a function of time to generate the concentration gradient eluent flow, in which the first generation chamber further includes a first volume and the second generation chamber further includes a second volume, and a sum of the first volume and the second volume ranges from about 1 microliter to about 100 microliters. |
