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1. A method for identifying a plant that is resistant to
i) a plant virus named Tomato Marchitez virus (ToMarV), deposited under depositors reference PRI-TMarV0601 on Jul. 10, 2007 with the Deutsche Sammlung von Mikroorganismen and Zellkulturen GmbH, in Braunschweig, Germany, the deposited material receiving the accession number DSM 19656, or ii) a Tomato Marchitez virus comprising at least one nucleic acid sequence selected from the group consisting of SEQ ID NO: 16 and a sequence having a nucleotide sequence homology of at least 80% thereto, said virus having isometric virus particles with a diameter of approximately 28 nm, having a viral genome consisting of two (+) ssRNA molecules of about 7.2 kb (RNA1) and about 4.9 kb (RNA2), wherein the viral capsid contains three coat proteins of approximately 35, 26 and 24 kDa, respectively, and wherein the virus induces severe leaf necrosis in tomato plants, beginning at the base of the leaflets, and necrotic rings on tomato fruits, the method comprising the steps of:
a) exposing a plant or plant part to an infective dosage of the virus by mechanical inoculation of virus particles or virus nucleic acid on plants, andb) identifying said plant as a resistant plant when, after said exposure, either
disease-symptoms in said plant or plant part remain absent or are delayed in expression or are at least reduced in severity or are localized relative to a susceptible control plant, and/orthe virus or genomic sequences thereof are not present in said plant or plant part or the presence of the virus is at least quantitatively reduced relative to a susceptible control plant. |
