Method of single point internal lock-mobility correction

摘要

A method of mass spectrometry is disclosed comprising passing ions through an ion mobility spectrometer and acquiring first ion mobility drift time data. A calibration function is applied to the first ion mobility drift time data to determine a physico-chemical property (e.g. CCS) of the ions. Second ion mobility drift time data is then acquired and the calibration function is applied to the second ion mobility drift time data to determine the physico-chemical property of one or more known or reference ions. The calibration function is then adjusted.

主权项

1. A method of mass spectrometry comprising:
separating a first plurality of ions including one or more known or reference ions according to their ion mobility and acquiring first ion mobility data; applying an initial calibration function to said first ion mobility data; determining an estimated property of said one or more known or reference ions; comparing said estimated property with a known property of said one or more known or reference ions; and varying said initial calibration function to generate a revised calibration function; wherein the step of varying said initial calibration function comprises altering one or more constants or one or more coefficients or one or more terms of said initial calibration function to generate said revised calibration function; wherein the step of varying said initial calibration function is performed in real-time during an experimental acquisition; and wherein the step of varying said initial calibration function comprises either: (a) altering a first order coefficient or a first order term in said initial calibration function; or (b) altering a second or higher order coefficient or a second or higher order term in said initial calibration function.