Soybean plant and seed corresponding to transgenic event MON87754 and methods for detection thereof

摘要

The present invention provides the transgenic soybean event MON87754, and the cells, seeds, plant parts, and plants comprising DNA diagnostic for this transgenic soybean event. The event itself functions to increase the oil content of soybeans carrying the event in their genome relative to commodity versions of soy. The invention also provides compositions comprising nucleotide sequences that are diagnostic for said soybean event in a sample, methods for detecting the presence of said soybean event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said soybean event in a sample, growing the seeds of such soybean event into soybean plants, and breeding to produce soybean plants comprising DNA diagnostic for the soybean event.

主权项

1. A method of detecting the presence of a soybean event MON87754 nucleotide sequence in a biological sample, the method comprising:
i. contacting the sample with a DNA primer pair of sufficient length of contiguous nucleotides of SEQ ID NO: 6 or its full complement, wherein said pair is capable of producing an amplicon diagnostic for event MON87754; ii. performing a nucleic acid amplification reaction, thereby producing an amplicon; and iii. detecting said amplicon, wherein said amplicon comprises SEQ ID NO: 1 or SEQ ID NO: 2.