| 发明名称 | Method for delivering agents into cells using bacterial toxins | ||
| 摘要 | The invention provides compositions and methods for delivering a bioactive moiety comprising at least one non-natural component into a cell cytosol of an eukaryotic cell. The bioactive moiety is linked to an A component of a bacterial toxin, a functional wild-type or modified fragment thereof, or an A component surrogate or mimetic. For delivery, the cell is contacted with the linked bioactive moiety and a corresponding B component of the bacterial toxin or a functional fragment thereof. | ||
| 申请公布号 | US9079952(B2) | 申请公布日期 | 2015.07.14 |
| 申请号 | US201213978550 | 申请日期 | 2012.01.10 |
| 申请人 | President and Fellows of Harvard College | 发明人 | Collier Robert J.;Pentelute Brad L. |
| 分类号 | C07K11/00;C12P21/08;C07K14/195;C07K16/46;C12N9/52;A61K38/00 | 主分类号 | C07K11/00 |
| 代理机构 | Nixon Peabody LLP | 代理人 | Nixon Peabody LLP ;Resnick David S.;Karttunen Contarino Leena H. |
| 主权项 | 1. A method for delivering a bioactive moiety comprising at least one non-natural component into a cell cytosol of an eukaryotic cell comprising contacting the cell with (a) a fusion molecule comprising the bioactive moiety attached to an A component of a bacterial toxin, a functional wild-type or modified fragment thereof, or an A component surrogate or mimetic; and (b) a corresponding B component of the bacterial toxin or a functional fragment thereof, wherein the non-natural component is selected from the group consisting of a peptide or protein comprising one or more D-amino acids. | ||
| 地址 | Cambridge MA US | ||