METHOD FOR PREPARING LIRAGLUTIDE

摘要

Disclosed is a method for synthesizing liraglutide. The method comprises the specific steps: A) synthesizing a segment Fmoc-Lys-(Glu(Nα-Palmitoyl)-OtBu)-OH by using a liquid phase manner; B) coupling a resin solid-phase carrier and Fmoc-Gly-OH, so as to obtain Fmoc-Gly-resin; C) sequentially coupling amino acids with N-end Fmoc protection and side chain protection according to a liraglutide main chain peptide sequence by using a solid-phase synthesis method, a lysine tripeptide segment using Fmoc-Lys-(Glu(Nα-Palmitoyl)-OtBu)-OH; and D) performing splitting, purification and freeze-drying to obtain liraglutide. The method needs a short synthesis period and low cost, produces high yield, and is suitable for large-scale production.