Measurement of autoantibodies at low conductivity with increased sensitivity

摘要

Methods for detecting or capturing low-avidity autoantibodies in a biological sample are provided. Target antigen used to assay for the low-avidity autoantibodies of interest is immobilized on a solid phase. The biological sample is contacted under low conductivity condition with the target antigen for which the autoantibodies has specific binding affinity. Binding of the target antigen to the autoantibodies of interest in the biological sample is then detected to ascertain the presence or concentration of the autoantibodies of interest.

主权项

1. A method for detecting an autoantibody in a biological sample, said autoantibody being specific for a target antigen, the method comprising the steps of:
(a) contacting the biological sample under a low conductivity condition with the target antigen for which the autoantibody has specific binding affinity; and (b) detecting the binding of said target antigen to said autoantibody in the biological sample, wherein said target antigen is immobilized on a solid phase and the presence of said binding is indicative of the autoantibody in the biological sample, and wherein the low conductivity condition has a conductivity of less than the conductivity of a 90 mM sodium chloride solution.