Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol with anti-tumor activity and preparation method thereof

摘要

The preparation of Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol with anti-tumor activity includes the following steps: 1) at normal temperature and under normal pressure, adding a Vitamin C solution to an aqueous solution of Pleurotus tuber-regium polysaccharides, and mixing uniformly; 2) adding dropwise a selenium dioxide solution or a selenite solution to the solution while mixing uniformly; and 3) adding water to the solution to a pre-determined volume, to obtain a Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol when the red color of the product is no longer deepened. The polysaccharides are used to control the particle size of nano-selenium, to obtain functionalized nano-elemental selenium with high anti-tumor activity and low toxicity. The anti-tumor activity of the biologically active molecule polysaccharides and the physiological effect of nano-selenium are combined, and a synergistic anti-tumor effect is achieved.

主权项

1. A Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol with anti-tumor activity, comprising:
Pleurotus tuber-regium polysaccharides, in a concentration range of 10 mg·L−1-1000 mg·L−1; Vitamin C, in a concentration range of 0.02 mmol·L−1-20 mmol·L−1; and nano-selenium, in a concentration range of 0.005 mmol·L−1-5 mmol·L−1; wherein the Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol is prepared by steps of:
Step 1) at a temperature in a range of 15-35° C., adding a Vitamin C solution to an aqueous solution of Pleurotus tuber-regium polysaccharides, and mixing uniformly;Step 2) adding dropwise a selenium dioxide solution or a selenite solution to the solution obtained in Step 1 while mixing uniformly; andStep 3) adding water to the solution obtained in Step 2 to a pre-determined volume, to obtain a Pleurotus tuber-regium polysaccharide functionalized nano-selenium hydrosol when the red color of the product is no longer deepened.