Fremgangsmåde til DNA-sekvensering med hybridisering
摘要
The present invention relates to a method for the determination of a nucleic acid sequence by physical manipulation. In particular, the said method comprises the steps of denaturing a double-stranded nucleic acid molecule corresponding to the said nucleic acid sequence by applying a physical force to the said molecule; and detecting a blockage of the renaturation of the double-stranded nucleic acid molecule. More specifically, the method comprises the steps of denaturing a double-stranded nucleic acid molecule corresponding to the said nucleic acid sequence by applying a physical force to the said molecule; providing a single-stranded nucleic acid molecule; renaturing the said double stranded nucleic acid molecule in the presence of the said single-stranded nucleic acid molecule; and detecting a blockage of the renaturation of the double-stranded nucleic acid.
申请公布号
DK2576818(T3)
申请公布日期
2015.07.06
申请号
DK20110721549T
申请日期
2011.05.26
申请人
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);UNIVERSITÉ PIERRE ET MARIE CURIE (PARIS 6);ECOLE NORMALE SUPÉRIEURE
发明人
BENSIMON, DAVID;ALLEMAND, JEAN-FRANÇOIS;MANOSAS, MARIA;DING, FANG-YUAN;CROQUETTE, VINCENT