Nucleic acid ligands against infectious prions

摘要

The invention generally relates to nucleic acid ligands that specifically bind to infectious prions, and methods of diagnosing a transmissible spongiform encephalopathy disease in a subject. In certain embodiments, the invention provides an isolated nucleic acid ligand that binds to an infectious prion. In other embodiments, the invention provides a method for diagnosing a transmissible spongiform encephalopathy disease in a subject including obtaining a tissue or body fluid sample from a subject, contacting the tissue or body fluid with a nucleic acid ligand that binds to an infectious prion, thereby detecting the infectious prion in the sample, and diagnosing the transmissible spongiform encephalopathy disease based on results of the contacting step.

主权项

1. A method for assessing whether a subject may be afflicted with a chronic wasting disease comprising:
providing a tissue or body fluid sample from a subject; contacting the tissue or body fluid with a nucleic acid ligand that distinguishes between an infectious prion associated with a chronic wasting disease and a non-infectious prion and binds to only the infectious prion, wherein the nucleic acid ligand consists of the nucleotide sequence of SEQ ID NO: 1 or the nucleotide sequence of SEQ ID NO: 2; detecting the bound nucleic acid ligand, thereby detecting the infectious prion in the sample; and assessing whether the subject may be afflicted with the chronic wasting disease based on results of the detecting step.