主权项 |
1. A method for reducing ethylene biosynthesis in a plant, comprising:
(a) introducing into a plant cell a recombinant expression cassette comprising a first polynucleotide operably linked to a heterologous promoter, wherein said expression cassette directs expression of said first polynucleotide, wherein the first polynucleotide is selected from the group consisting of:
i. a polynucleotide comprising the full length nucleotide sequence of SEQ ID NO: 1, 3, 5, or 7;ii. a polynucleotide having at least 95% sequence identity to the full length of the sequence set forth in SEQ ID NO: 1, 3, 5, or 7, wherein the polynucleotide encodes a polypeptide having ethylene over-producer 1 (ETO1) activity and wherein the polypeptide comprises the N-terminal domain provided as SEQ ID NO: 11 and the C-terminal domain provided as SEQ ID NO: 12; andiii. a polynucleotide encoding a polypeptide comprising the full length amino acid sequence set forth in SEQ ID NO: 2, 4, 6, or 8; and (b) culturing the plant cell under plant cell growing conditions and regenerating a plant therefrom, wherein the level of ethylene biosynthesis in said plant is reduced, relative to a control. |