发明名称 |
METHODS AND COMPOSITIONS FOR SILENCING GENES USING ARTIFICIAL MICRORNAS |
摘要 |
Methods and compositions are provided that employ microRNA (miRNA) that, when expressed in a plant cell, is capable of reducing the level of mRNA of a target sequence (i.e. endogenous sequence) without reducing the level of mRNA of one or more closely related sequences. While miRNAs can be designed with specificity for a particular target sequence, the instant application demonstrates that a miRNA can specifically silence a target sequence without silencing a closely related sequence having high sequence identity to the target sequence. In certain embodiments, an endogenous target sequence can be suppressed with a recombinant miRNA expression construct without silencing a recombinant polynucleotide of interest having a sequence closely related to the target sequence. Such methods and compositions employ recombinant miRNA expression constructs which produce a 21-nt miRNA. Transgenic plant cells, plants and seeds incorporating miRNA expression constructs and recombinant polynucleotide constructs comprising polynucleotides of interest are also provided. |
申请公布号 |
ZA201401942(B) |
申请公布日期 |
2015.06.24 |
申请号 |
ZA20140001942 |
申请日期 |
2014.03.17 |
申请人 |
E.I. DU PONT DE NEMOURS AND COMPANY;PIONEER HI-BRED INTERNATIONAL, INC |
发明人 |
KUREK, ITZHAK;ZHU, GENHAI;MCGONIGLE, BRIAN |
分类号 |
C12N |
主分类号 |
C12N |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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