Nanopore sequencing using replicative polymerases and helicases
摘要
The present invention relates to a system of nanopore sequencing using nucleic-acid binding proteins wherein the activity of the said protein and the passage of the sequenced nucleic acid molecule through the nanopore are coordinated. In particular, the present invention enables an easy synchronization of nanopore capture with polynucleotide unwinding, which in turn affords an easy way to repeat the whole process of capture and nucleotide characterization.
申请公布号
EP2886663(A1)
申请公布日期
2015.06.24
申请号
EP20130306795
申请日期
2013.12.19
申请人
CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS);ECOLE NORMALE SUPÉRIEURE;UNIVERSITE D'EVRY VAL D'ESSONNE