IMMUNOASSAY-BASED DETERMINATION OF IN-SOLUTION BINDING KINETICS

摘要

Herein is reported a method for the determination of the binding affinity of a binder and its ligand comprising the step of determining based on the result of an immunoassay the fraction of free binder in a sample comprising binder, ligand and binder-ligand-complexes for at least two different binder:ligand ratios in the sample, and if the determined fraction of free binder is not comparable for all used binder:ligand ratios then the binder:ligand ratio in the sample is lowered and the sample is re-analyzed by the same immunoassay, and calculating based on the fraction of free binder in the previous step the binding affinity for the binder to its ligand.

主权项

1. A method for the determination of the binding affinity of a drug to its target comprising the following step:
determining the fraction of free drug or free target in a sample comprising drug, target and non-covalent drug-target-complexes in the free drug plateau, and calculating based on the fraction of free drug or calculating based on the fraction of free target determined in the previous step the binding affinity for the drug to its target.