METHOD FOR LOADING OF DENDRITIC CELLS WITH CLASS I ANTIGENS

摘要

Disclosed is a method to obtain dendritic cells loaded with an exogenously added antigen. The method is in particular applicable to human cell lines that may be used in the production of dendritic cell vaccines, e.g. to be used in the treatment of cancer. In the method the antigen to be loaded on the cells is added to the cells at particular stages of the development of the dendritic cells. A pharmaceutical composition comprising the obtained cells is also disclosed.

主权项

1. A method for obtaining mature dendritic cells loaded with an HLA Class I antigenic peptide, the method comprising:
cultivating immature dendritic cells in a culture medium in the presence of at least one compound that is capable of inducing maturation of the immature dendritic cells, and wherein during at least part of the cultivating at least one exogenously added HLA Class I antigenic peptide is present in the culture medium, wherein the peptide consists of 8, 9, 10 or 11 amino acids, and collecting the mature dendritic cells loaded with an HLA Class I antigenic peptide.