Cell selection method and cells obtained therefrom

摘要

The present invention relates to a method for the in vitro selection of at least one therapeutic cell subpopulation from an original cell population of eukaryotic cells, as well as to the therapeutic cell subpopulation selected by the method, which can be used for the treatment of diseases of tissues or organs. The selected cells are highly migrative and the method according to the invention provides for the selection of these highly migrative subpopulations from a sample comprising a mixture of cells.

主权项

1. A method for the in vitro selection of at least one therapeutic cell subpopulation from an original cell population of eukaryotic cells, wherein the therapeutic cell subpopulation can be used for the treatment of diseases of tissues or organs, and wherein the method has the following consecutive steps:
a) Addition of an original cell population to a first starting reservoir, which is connected via at least one selection means having at least one opening to at least one second selection reservoir, wherein the at least one opening of the at least one selection means has a diameter of 3 μm to 500 μm; b) Addition of at least one cell culture medium to the at least one selection reservoir to which the therapeutic cell subpopulation migrates; c) Culturing of the original cell population for a period of 15 min to 48 hours, within which period a cell subpopulation migrates through the at least one opening of the selection means towards the at least one selection reservoir; and d) Selection and obtaining of a cell, which is selected from at least one of the cells that migrated as the first to the one hundred thousandth cell through the at least one opening of the selection means into the at least one selection reservoir, in order to obtain a cell subpopulation with an elevated migration potential or tropism to a certain cell type compared to the original cell population.