Method for producing a target substance by fermentation

摘要

A target substance can be produced by culturing a bacterium having an ability to produce 2-ketoglutaric acid or a derivative thereof, and an ability to produce xylonic acid from xylose, which is imparted with xylonate dehydratase activity, 2-keto-3-deoxyxylonate dehydratase activity and 2-ketoglutaric semialdehyde dehydrogenase activity, or in which these activities are enhanced, in a medium containing xylose as a carbon source to produce and accumulate the target substance in the medium, and collecting the target substance from the medium.

主权项

1. A method for producing a target substance comprising
A) culturing a bacterium belonging to the genus Pantoea or the genus Corynebacterium and having an ability to produce the target substance in a medium containing xylose wherein the bacterium is able to produce the target substance through conversion of xylonic acid produced from xylose into 2-ketoglutaric acid by xylonate dehydratase, 2-keto-3-deoxyxylonate dehydratase, and 2-ketoglutaric semialdehyde dehydrogenase, B) allowing the bacterium to produce and secrete the target substance into the medium, and C) collecting the target substance from the medium, wherein the target substance is 2-ketoglutaric acid or a derivative thereof, the bacterium has an ability to produce xylonic acid from xylose, and activities of the enzymes xylonate dehydratase, 2-keto-3-deoxyxylonate dehydratase, and 2-ketoglutaric semialdehyde dehydrogenase have been imparted to or enhanced in the bacterium; wherein the 2-ketoglutaric acid derivative is selected from the group consisting of L-glutamic acid, L-glutamine, L-arginine, L-citrulline, L-ornithine, L-proline, putrescine, and γ-aminobutyric acid, wherein the bacterium can produce xylonic acid from xylose because of any one of the following characteristics: (i) xylose dehydrogenase activity, or xylose dehydrogenase activity and xylonolactonase activity have been imparted to or enhanced in the bacterium, or (ii) the bacterium has glucose dehydrogenase activity that can catalyze a reaction producing xylonic acid from xylose, wherein said activities are imparted to or enhanced in the bacterium by introducing expressable forms of genes coding for the enzymes into the bacterium, wherein the genes are native to a microorganism belonging to a genus selected from the group consisting of Caulobacter, Escherichia, Agrobacterium, Herbaspirillum, Actinoplanes, Cupriavidus, Pseudomonas, Zobellia, Thermobacillus, Arthrobacter, Azospirillum, Halomonas, Bacillus, and Aspergillus.