Monoclonal antibodies against GMF-B antigens, and uses therefor

摘要

The disclosure relates to anti-glial maturation factor beta (“GMF-B”) monoclonal antibodies (mAbs) and fragments thereof, as well as hybridoma lines that secrete antibodies or fragments. Therapeutic and diagnostic uses of such antibodies, including treatment and detection of cancer and dementia, and methods and kits for detecting cells or samples expressing GMF-B, including soluble GMF-B, are also encompassed.

主权项

1. A method for diagnosing breast cancer in a human subject comprising:
a) contacting a tissue or cell sample from a human with an isolated antibody or antibody fragment specific for glia maturation factor beta (GMF-B), wherein the antibody or antibody fragment comprises a heavy chain variable domain comprising three complementarity determining regions (CDRs) comprising the amino acid sequences of SEQ ID NO: 2, SEQ ID NO: 3, and SEQ ID NO: 4, and a light chain variable domain comprising three CDRs comprising the amino acid sequences of SEQ ID NO: 6, SEQ ID NO: 7, and SEQ ID NO: 8; b) detecting the antibody; and c) comparing the detection to a control sample from a healthy subject, wherein the subject is diagnosed with cancer if the antibody is detected at levels that are increased compared to the healthy control.