THREE-DIMENSIONAL FOCUSING DEVICE AND METHOD FOR A MICROSCOPE

摘要

The invention relates to a device (II) for three-dimensional focusing in a microscope (I) with a given resolution, comprising: - a detector (119) making it possible to determine, in a given measuring plane, a phase and intensity image of an electromagnetic field resulting from the interaction of an incident light wave with a reference object (105) situated in a reference medium with a given index and having a complex refractive index, the real part and/or the imaginary part of which differs from the real part and/or the imaginary part, respectively, of the refractive index of the reference medium; - computation means (121) making it possible to determine a three-dimensional position variation of the reference object (105) from at least one phase and intensity image.