COMPOSITION, METHOD AND KIT FOR REDUCING BACKGROUND STAINING

摘要

Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups.

主权项

1. A blocking solution for reducing background staining, comprising:
a polymeric material comprising multiple carboxylate, sulfate, sulfonate, phosphate, or phosphonate groups, wherein, if the polymeric material is a poly(amino acid), the polymeric material comprises a sulfated, sulfonated or phosphonated poly(amino acid), poly(aspartic acid) or poly(glutamic acid); and a buffer or water.