发明名称 |
FAST THERMO-OPTICAL PARTICLE CHARACTERISATION |
摘要 |
The present invention relates to a method and an apparatus for a fast thermo-optical characterisation of particles. In particular, the present invention relates to a method and a device to measure the stability of (bio)molecules, the interaction of molecules, in particular biomolecules, with, e.g. further (bio)molecules, particularly modified (bio)molecules, particles, beads, and/or the determination of the length/size (e.g. hydrodynamic radius) ofindividual (bio)molecules, particles, beads and/or the determination of length/size (e.g. hydrodynamic radius). |
申请公布号 |
US2015125897(A1) |
申请公布日期 |
2015.05.07 |
申请号 |
US201414504843 |
申请日期 |
2014.10.02 |
申请人 |
Ludwig Maximilians Universität München |
发明人 |
DUHR Stefan;Baaske Philipp |
分类号 |
G01N21/64;G01K11/12;G01N15/14 |
主分类号 |
G01N21/64 |
代理机构 |
|
代理人 |
|
主权项 |
1. A method for measuring inter- and/or intra-molecular interactions of particles in a solution comprising:
a. providing a sample with marked particles in a solution; b. exciting fluorescently the marked particles and detecting a first fluorescence of the excited particles; c. irradiating a laser light beam into the solution to obtain a spatial temperature distribution in the solution around the irradiated laser light beam; d. detecting a second fluorescence of the particles in the solution at a predetermined time after irradiation of the laser into the solution has been started, and e. characterizing the inter- and/or intra-molecular interactions of the particles based on the first and second detections, wherein the sample with marked particles in a solution is in chamber in a multi-well plate. |
地址 |
Munchen DE |