CLASS OF CYANO-SUBSTITUTED ASYMMETRIC CYANINE DYES, SYNTHESIZING METHOD AND APPLICATION THEREOF

摘要

The present invention provides a category of cyano-substituted asymmetric cyanine dyes having the following general structural Formula I and its synthesizing method. The cyano-substituted asymmetric cyanine dyes in present invention are easily synthesized and have long emission wavelength, high molar extinction coefficient, high sensitivity, good light stability, high fluorescence quantum yield after binding with nucleic acid, and low cell toxicity, which is beneficial for application as fluorescent dyes and could also be used in the field of identifying nucleic acid molecules, clinical diagnostics, and immunoassay testing etc.;

主权项

1. A cyano-substituted asymmetric cyanine dyes of Formula I: wherein R1 and R2 are each independently selected from the group consisting of H, C1-18 saturated/unsaturated alkyl, OR5 and halogen; R3 and R4 are each independently selected from the group consisting of C1-6 saturated/unsaturated alkyl and —CH2CH2OR5; R5 is selected from the group consisting of H and C1-6 alkyl; Y− is a halogen ion.