| 摘要 |
<p>Disclosed is a method for detecting the presence or level of an autoantibody to an anti-TNFαdrug in a sample without interference from the anti-TNFαdrug in the sample, the method comprising: a) contacting the sample with an acid to dissociate preformed complexes of the autoantibody and the anti-TNFαdrug, wherein the sample has or is suspected of having an autoantibody to the anti-TNFαdrug; b) contacting the sample with a labelled anti-TNFαdrug following dissociation of the preformed complexes; c) neutralising the acid in the sample to form labelled complexes of the labelled anti-TNFαdrug and the autoantibody; d) subjecting the labelled complexes to size exclusion chromatography to separate the labelled complexes; and e) detecting the labelled complexes, thereby detecting the presence or level of the autoantibody without interference from the anti-TNFαdrug in the sample.</p> |
