发明名称 NUCLEIC ACID-BASED AUTHENTICATION CODES
摘要 This invention relates to a nucleic-acid based product authentication by determining authentication codes comprising target nucleic acids using oligonucleotide probes immobilized on particulate and non-particulate substrates. The presence of the authentication code is determined using detection methods, such as flow cytometric methods, capable of particle discrimination based on the light scattering or fluorescence properties of the particle, or by spatial resolution of oligonucleotides immobilized at specific loci on a substrate. Target-correlated fluorescence signal, originating from a target nucleic acid hybridized to substrate-immobilized oligonucleotide is determined as an indicator of the presence of the authentication code.
申请公布号 US2015111780(A1) 申请公布日期 2015.04.23
申请号 US201414563450 申请日期 2014.12.08
申请人 AUTHENTIFORM TECHNOLOGIES, LLC 发明人 Mercolino Thomas J.
分类号 C12Q1/68;G01N33/58 主分类号 C12Q1/68
代理机构 代理人
主权项 1. A method for authenticating a product by determining the presence of an authentication code comprising target nucleic acid, the method comprising the steps of: receiving the product to be authenticated, wherein the product has associated therewith the authentication code comprising one or more target nucleic acids, wherein each target nucleic acid is immobilized onto a microparticle, and wherein the microparticle comprises at least one first reporter compound capable of producing a distinct fluorescence signal corresponding to the microparticle and is capable of scattering electromagnetic radiation of wavelength greater than or equal to about 200 nm; contacting the one or more microparticles with one or more oligonucleotide probes comprising a nucleic acid sequence complementary to at least a portion of the one or more target nucleic acids to form a duplex heterodimer; and with a second reporter compound which upon binding or being bound to the duplex heterodimer is capable of producing a second detectable signal distinguishable from that of the first reporter compound; and determining the presence of the authentication code by detecting the one or more microparticles and the second detectable signal associated therewith.
地址 CHAPEL HILL NC US