Method for manufacturing a fat-soluble bioactive substance

摘要

Provided is a production method of a lipophilic bioactive substance, which includes mixing an aqueous suspension of a microbial cell containing the lipophilic bioactive substance or a microbial cell homogenate thereof and an organic solvent in the presence of a particular surfactant, and extracting the lipophilic bioactive substance into the organic solvent phase.;This production method enables extraction without using special dehydrating, drying facility, and without causing a decrease in the yield due to degraded separability between solvent and fungus body component, as well as efficient industrial production.

主权项

1. A production method of a lipophilic bioactive substance, comprising
mixing an aqueous suspension of a microbial cell containing a lipophilic bioactive substance or a microbial cell homogenate thereof with an organic solvent in the presence of at least one kind of non-ionic surfactant selected from the group consisting of polyoxyethylene-polyoxypropylene block copolymer surfactants, sucrose fatty acid esters, glycerol fatty acid esters, sorbitan fatty acid esters, polyetherpolyol surfactants, polyoxyethylene alkyl ether surfactants and alkylether surfactants, wherein the amount of the non-ionic surfactant is 0.01 to 10 wt % of the aqueous suspension of the microbial cell or microbial cell homogenate, and extracting the lipophilic bioactive substance.