PROCESS FOR THE SELECTIVE REDUCTION OF BILE ACIDS, THEIR SALTS OR DERIVATIVES, IN A BIPHASIC SYSTEM

摘要

New process for the stereo- and regioselective enzymatic reduction of bile acids, their salts or derivatives, such as 7-keto-lithocholic acid, 7- ketodesoxycholic acid methyl ester, or 7,12 diketolithocholic acid methyl ester, in a buffer/organic solvent biphasic system by the action of NAD (P) H-dependent 7beta-hydroxysteroid dehydrogenase (7beta- HSDH) in the presence of a glucose dehydrogenase (GDH) and glucose as co- substrate for the regeneration of the NAD ( P) H cofactor.