| 摘要 |
<p>Abstract The invention relates to a process for the amplification of nucleic acids by means of a polymerase chain reaction, in which a cycle consisting of the steps denaturing, annealing and elongation is performed repeatedly. In one embodiment, the gain (g) of copies of a nucleic acid to be amplified at the end of at least one of the passes of the cycle is less than 80 percent of the copies of the nucleic acid that were present at the start of that pass, and in at least one of the passes of the cycle a time of action (tA) is shorter than one second. In addition, in a further embodiment, the number (k) of passes of the cycle of the polymerase chain reaction is greater than 45 and/or in at least one of the passes a cycle time t, is shorter than 20 seconds. u/ co/ cc-T it U" I-'</p> |
