Process for the purification of high-purity Rubusoside

摘要

The invention provides a process of purifying Rubusoside from the Rubus suavissimus S. Lee plant extract. The process is useful for producing high purity Rubusoside with purity greater than 95% (dry basis). High purity rubusoside is useful as in combination with other caloric and non-caloric sweeteners as well as non-caloric sweetener in various food and beverage compositions. The high purity rubusoside is useful as non-caloric sweetener in edible and chewable compositions such as any beverages, confectioneries, bakeries, cookies, chewing gums, and alike.

主权项

1. A process for purifying Rubusoside from Rubus suavissimus extract comprising the steps of:
a. providing an extract of Rubus suavissimus S. Lee leaves; b. dissolving the extract in a first aqueous alcoholic solution to obtain a first mixture; c. inducing crystallization in the first mixture by temperature gradient treatment; d. filtering the first mixture from (c) to obtain a first precipitate and a first filtrate; e. dissolving the first precipitate in a second aqueous alcoholic solution to obtain a second mixture; f. adding activated carbon to second mixture and filtering it to obtain a decolorized second mixture; g. inducing crystallization in the decolorized second mixture by temperature gradient treatment; h. filtering the decolorized second mixture from (g) to obtain a second precipitate and a second filtrate; i. suspending the second precipitate in a third aqueous alcoholic solution to obtain a third mixture; j. filtering the third mixture to obtain a third precipitate and a third filtrate; and k. drying the third precipitate to yield purified Rubusoside.