摘要 |
Sparging apparatus and method for a reaction vessel for carrying out a cell culture, comprises conduit means (1) for conveying gas into the vessel and two groups of outlet apertures (4, 6) through which, in use, gas travels from the conduit means into liquid in the vessel to form bubbles in the liquid. Each group of apertures (4, 6) is situated in a respective zone on the conduit means, the zones being separated from each other so as to prevent or inhibit the coalescence of bubbles emitted through one group of apertures with those emitted through the other group of apertures. The conduit means (1) may be a pipe, each group of apertures being arranged in a respective row along the pipe. |