| 主权项 |
1. A process for preparing an amine product of structural formula (I): having the indicated stereochemical configuration at the stereogenic center marked with an *; in an enantiomeric excess over the opposite enantiomer, wherein R1 is optionally substituted aryl or heteroaryl; R2 is an optionally substituted C1-C6 alkyl, —R3C(O)R4, or —R3OC(O)R5; R3 is an optionally substituted C1-C4 alkyl; and R4 is H, an optionally substituted C1-C4 alkyl, NR6R7, or OR8, wherein R5, R6, R7, and R8 are independently H or C1-C4 alkyl; the process comprising contacting a ketone substrate of structural formula (II): with a transaminase polypeptide in presence of an amino donor under reaction conditions suitable for converting the ketone substrate to the amine product, wherein the transaminase polypeptide has at least 80% sequence identity to SEQ ID NO:74 and is capable of converting the ketone substrate to the amine product at a rate that is improved as compared to SEQ ID NO:2. |
