Fungal beta-xylosidase variants

摘要

The present invention provides fungal xylanase and/or beta-xylosidase enzymes suitable for use in saccharification reactions. The present application further provides genetically modified fungal organisms that produce xylanase and/or beta-xylosidases, as well as enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to fermentable sugars, enzyme mixtures produced by the genetically modified fungal organisms, and methods for producing fermentable sugars from cellulose using such enzyme mixtures.

主权项

1. A recombinant beta-xylosidase variant and/or biologically active recombinant beta-xylosidase variant fragment comprising at least one amino acid sequence comprising at least 70% sequence identity to SEQ ID NO:2 and comprising at least one mutation at position 31, 108, 115, 174, 177, 203, 209, 211, 219, 235, 264, 280, 309, 320, 322, 345, 347, 375, 379, 389, 394, 398, 431, 438, 449, 475, 482, 484, 499, 525, 539, 560, 565, 571, 572, 589, 662, 727, 761, 763, 798, and/or 842, wherein the positions are numbered with reference to SEQ ID NO:2, and wherein said recombinant beta-xylosidase variant comprises SEQ ID NO:27.