BIFUNCTIONAL FUSION PROTEIN, PREPARATION METHOD THEREFOR, AND USE THEREOF

摘要

Provided are a bifunctional fusion protein having a CTLA4 molecule extracellular region, an Fc fragment, and a functional fragment that neutralizes TNFαactivity, a gene for encoding the bifunctional fusion protein, a carrier having the gene, a host cell having the carrier, and a pharmaceutical composition having the bifunctional fusion protein. The functional fragment that neutralizes TNFαactivity is a TNFR2 molecule extracellular region or an anti-TNFαsingle-chain antibody, wherein the CTLA4 molecule extracellular region is located at the N-terminus of the bifunctional protein, and the TNFR2 molecule extracellular region or the anti-TNFαscFv is located in the middle or the C-terminus of the bifunctional protein. The bifunctional fusion protein can simultaneously block the signal pathways of B7/CD28 and TNFα/TNFR, thereby inhibiting inflammation reactions. Comparing to single-function proteins, the present invention can lower the production cost, reduce clinically administered medicine volume and frequency, and enhance compliance of the test subjects.