METHOD OF INTRACELLULAR INFECTIOUS AGENT DETECTION IN SPERM CELLS

摘要

The present invention describes a method for investigating the presence of chlamydia, viruses and other infectious agents in spermatozoa, with the use of immunofluorescence in combination with flow cytometry. This method is used for the intracellular detection of microorganisms within sperm cells through the use of a DNA easing procedure, as well as for the detection of microorganisms adherent to the surface of spermatozoa.

主权项

1. Method for the investigation of the presence of viruses, chlamydia, parasites and other infectious pathogens intracellularly in spermatozoa, which method comprises the following steps:
A procedure for the easing of the dense structure of the DNA of the sperm cells including spermatozoa, direct or indirect intra-sperm cell immunofluorescence, visualization and evaluation of results by flow cytometry, and which method is characterized by the fact that the step of easing of the dense structure of the DNA must necessarily take place before immunofluorescence.