| 摘要 |
PROBLEM TO BE SOLVED: To provide encapsulation or non-encapsulation cell remedies, which can regulate protein secretion.SOLUTION: The present invention relates to compositions for treating, ameliorating, or preventing a disease or disorder, comprising the following (1) and (2): (1) a first polynucleotide encoding a gene switch, wherein the gene switch comprises at least one transcription factor sequence encoding a ligand-dependent transcription factor through operable connection with a therapeutic switch promoter, and wherein the therapeutic switch promoter is activated under conditions associated with a disease or disorder but is not a stress-inducible promoter; and (2) a second polynucleotide encoding a therapeutic polypeptide or therapeutic polynucleotide operably connected with a factor-regulated promoter which is activated by the ligand-dependent transcription factor, wherein the ligand can induce the expression of a therapeutic polypeptide or therapeutic polynucleotide. Preferably the polynucleotide is encapsulated in a microcapsule and the like. |
