METHOD AND APPARATUS FOR SAMPLE INJECTION IN LIQUID CHROMATOGRAPHY

摘要

The present invention provides a method and apparatus for substantially eliminating destructive transients of pressure or flow rate which can degrade the efficiency and useful lifetime of chromatography columns. The present invention enables a substantially constant flow of mobile phase liquid to be maintained through the chromatography system by eliminating the flow blockage interval associated with the actuation of sample injection valves. The present invention further provides a method to reduce the pressure and flow rate transients associated with pressurization of the sample loop contents when the sample loop is introduced to chromatography system delivery pressure.

主权项

1. A rotary shear-seal injection valve comprising:
a stator having a plurality of stator ports, at least one of said stator ports being in fluid communication with a pump; and a rotor rotatably abutting said stator, said rotor having a plurality of channels fluidly connecting at least two of the stator ports, the plurality of channels and plurality of stator ports being configured to maintain a first flow path from the pump to a column during a valve transition from a load state to an inject state while establishing a second flow path through the valve between a syringe port and a sample needle port.