Cellulase production method based on the regulation of the dissolved oxygen pressure oscillation in the culture medium

摘要

The present invention relates to a method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated by an oscillation of the dissolved oxygen partial pressure in the medium.

主权项

1. A method of producing cellulolytic and/or hemicellulolytic enzymes by a cellulolytic microorganism in a stirred and aerated bioreactor, comprising a growth stage in the presence of a carbon source and a production stage in the presence of a carbon-containing inductive substrate, wherein the supply of carbon-containing inductive substrate during the production stage is regulated according to the dissolved oxygen partial pressure in the growth medium, said pressure oscillating between two values Po2min and Po2max, the value of Po2min being greater than the critical pressure PO2critical below which the activity of the microorganism is affected and less than 95% of the saturation oxygen partial pressure Po2sat set when starting the bioreactor, and the value of Po2max being at least 5% greater than Po2min, addition of the carbon-containing inductive substrate being started as soon as the dissolved oxygen partial pressure in the medium is greater than value Po2max and stopped as soon as the dissolved oxygen pressure in the medium is below value Po2min.